Investigation of Phytocomponents and Gc-Ms Analysis of Ethanolic Extract of Sargassum Polycystum C.Agradh
Velayutham Mani Mala1, Ponnudurai Pon Malar2, P. Essly Selva Jasmine3
1Velayutham Mani Mala, Department of Pharmaceutical Chemistry, St.Mariam College of Pharmacy, Tirunelvelli, Pudur (Tamil Nadu), India.
2Ponnudurai Pon Malar, Department of Pharmaceutices, St.Mariam College of Pharmacy, Tirunelvelli, Pudur (Tamil Nadu), India.
3P. Essly Selva Jasmine, Department of Pharmacology, St.Mariam College of Pharmacy, Tirunelvelli, Pudur (Tamil Nadu), India.
Manuscript received on 09 January 2023 | Revised Manuscript received on 24 January 2023 | Manuscript Accepted on 15 February 2023 | Manuscript published on 28 February 2023 | PP: 29-32 | Volume-3 Issue-2, February 2023 | Retrieval Number: 100.1/ijapsr.C4010043323 | DOI: 10.54105/ijapsr.C4010.03020223
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Abstract: Aim: To qualitatively analyse the chemical composition in ethanolic extract from the whole part of Sargassum polycystum C.Agradh. by gas chromatography – mass spectroscopy (GC-MS). Methods: The dried entire part of Sargassum polycystum C. agardh was filtered after completion of cold maceration using a muslin cloth and Whatman filter paper, and an ethanolic extract was obtained using a cold maceration extractor. Results: The solubility level of different solvents in ethanol, chloroform, and methanol was higher in Sargassum polycystum. Then hexane, ethyl acetate, benzene and acetone were sparingly soluble in Sargassum polycystum. The physicochemical parameters of Sargassum polycystum dried were found to be total ash (6.74% w/w), water-soluble ash (1.23% w/w), acid-soluble ash (2.24% w/w), and loss on drying (5.99% w/w), which were also determined. The phytochemical screening of Sargassum polycystum C. Agardh reveals the presence of various phytochemical compounds, including alkaloids, terpenoids, flavonoids, cardiac glycosides, lipids, carbohydrates, and steroids—the absence of phytochemical compounds such as proteins, saponins, tannins, volatile oil and phenols. GC-MS analysis detected three active components in the ethanolic extract [1]. The identification of phytochemical compounds is based on retention time, molecular formula, peak area, and molecular weight, as presented.
Keywords: Ethanolic extract, GC-MS analysis, Physicochemical Components, and Sargassum Polycystum C. Agardh.
Scope of the Article: Pharmacy Practice